Human dental pulp stem cells cultured in serum-free supplemented medium

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Human dental pulp stem cells cultured in serum-free supplemented medium

UNLABELLED Growing evidence show that human dental pulp stem cells (DPSCs) could provide a source of adult stem cells for the treatment of neurodegenerative pathologies. In this study, DPSCs were expanded and cultured with a protocol generally used for the culture of neural stem/progenitor cells. METHODOLOGY DPSC cultures were established from third molars. The pulp tissue was enzymatically d...

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Transdifferentiation of Human Dental Pulp Stem Cells Into Oligoprogenitor Cells

Introduction: The nerve fibers in central nervous system are surrounded by myelin sheet  which is formed by oligodendrocytes. Cell therapy based on oligodendrocytes and their precursors transplantation can hold a promising alternative treatment for myelin sheet repair in demyelinating diseases. Methods: Human Dental Pulp Stem Cells (hDPSCs) are noninvasive, autologous and easy available s...

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Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teet...

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Characterization of dental pulp stem cells from impacted third molars cultured in low serum-containing medium.

We isolated and expanded stem cells from dental pulp from extracted third molars using an innovative culture method consisting of low serum-containing medium supplemented with epidermal growth factor and platelet-derived growth factor BB. We evaluated the differentiation potential of these cells when they were growing either adherently or as micromass/spheroid cultures in various media. Undiffe...

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Characterization of human dental pulp cells grown in chemically defined serum-free medium

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ژورنال

عنوان ژورنال: Frontiers in Physiology

سال: 2013

ISSN: 1664-042X

DOI: 10.3389/fphys.2013.00357